In the following you will obtain a brief overview of the PGx GenoChip technology. If you would like any further information please contact us directly.
The GenoChip is permanently integrated onto the bottom of a 1.5 mL reaction tube. The base area of the GenoChip spans only 3x3 mm and contains sequence-specific oligonucleotide probes or antibodies depending on the application. The chip layout that is the positions of the individual probes/antibodies can be customized. Up to 15x15 spots are possible for the GenoChip. All development and production steps are subject to an intensive quality management system which allows the application of the PGx GenoChip platform as IVD in the first place.
Layout and format
The GenoChip Image Reader is a compact and flexible read-out device.
To allow a simple and cost-effective multiplex detection, the robust method of precipitation staining was refreshed. The procedure, based on the ELISA technique, is applicable universally both to DNA (oligonucleotides) and to serology (antibody) based applications. It is achieved by an enzymatically initiated precipitation reaction of a dye which is directly proportional to the amount of bound target.
A light-transmission measurement allows the read-out of the resulting precipitation pattern with the GenoChip Image Reader.
Step 1: Hybridization
Incubation of the sample on the GenoChip. Meanwhile the assay-specific binding of targets to the probes takes place.
Step 2: Conjugation
Labeling of bound target molecules by adding a streptavidin-enzyme conjugate
Step 3: Precipitation
Addition of the substrate. The streptavidin-enzyme complex bound to the target molecules initiates the cleavage of the substrate into a colored precipitate. The resulting pattern of the enzymatic reaction is read out with a CCD-based reader and subsequently evaluated.
The precipitation reaction is characterized by an easy-to-handle and robust method with stable signals and no quencher effects. All reagents are non-toxic and inexpensive.